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anti mouse cd8 α  (Bio X Cell)


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    Structured Review

    Bio X Cell anti mouse cd8 α
    Anti Mouse Cd8 α, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd8/InVivoMAb+anti-mouse+CD8%CE%B2/pm42829916-411-4-19
    Average 96 stars, based on 204 article reviews
    anti mouse cd8 α - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    In Vivo:

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. In vivo CD8+ T cell depletion: anti-mouse CD8 (BE0061, BioXcell), and anti-rat IgG2b isotype control (BE0090, BioXcell). .. In vivo anti-PD1 treatment: anti-mouse PD1 (BE0273, BioXcell) and anti-rat IgG2a isotype control (BE0089, BioXcell).

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. In vivo CD8 + T cell depletion: anti-mouse CD8 (BE0061, BioXcell), and anti-rat IgG2b isotype control (BE0090, BioXcell). .. In vivo anti-PD1 treatment: anti-mouse PD1 (BE0273, BioXcell) and anti-rat IgG2a isotype control (BE0089, BioXcell).

    Article Title: PARP inhibitors restore NK cell function via secretory crosstalk with tumor cells in prostate cancer
    Article Snippet: At 18 to 20 days after tumor injection, 150 mg/kg of d -luciferin, potassium salt (Yeasen) was injected into the abdominal cavity of the mice, and in vivo imaging analysis was performed 10 minutes after injection into the body. .. For in vivo depletion of immune cells, 200 μg per mouse of anti-mouse NK-1.1 (Bio X Cell) and anti-mouse CD8 (Bio X Cell) antibodies were administered intraperitoneally 2 days before tumor injection, followed by injections every 3 days. .. For in vivo CypA blockade, anti-CypA mAb (BGI Genomics) was intraperitoneally administered at a dose of 200 μg per mouse on day 1 after tumor inoculation, followed by weekly injections thereafter ( ).

    Control:

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. In vivo CD8+ T cell depletion: anti-mouse CD8 (BE0061, BioXcell), and anti-rat IgG2b isotype control (BE0090, BioXcell). .. In vivo anti-PD1 treatment: anti-mouse PD1 (BE0273, BioXcell) and anti-rat IgG2a isotype control (BE0089, BioXcell).

    Article Title: TROP2/claudin program mediates immune exclusion to impede checkpoint blockade in breast cancer.
    Article Snippet: .. For CD8+T cell depletion, each mouse was administered intraperitoneally 200 μg anti- mouse CD8 (Bio X Cell, BE0061) or IgG2b isotype control (Bio X Cell, BE0090) 1 day before tumor inoculation and two times per week thereafter. .. Immunoprecipitation was performed using Pierce Classic Magnetic IP Kit (Thermo Scientific, 88804) with minor modifications.

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. In vivo CD8 + T cell depletion: anti-mouse CD8 (BE0061, BioXcell), and anti-rat IgG2b isotype control (BE0090, BioXcell). .. In vivo anti-PD1 treatment: anti-mouse PD1 (BE0273, BioXcell) and anti-rat IgG2a isotype control (BE0089, BioXcell).

    Article Title: Targeting tumor intrinsic TAK1 engages TNF-α-driven cell death through distinct mechanisms and enhances cancer immunotherapy
    Article Snippet: Where indicated, mice were treated with anti-mouse PD-1 antibody synthesized at AbbVie (17D2[mu IgG2a/k] DANA) 10 mg/kg, Q4Dx3, IP). .. For the CD8 depletion protocol, mice were injected with anti-mouse CD8 (BioXCell; BP0061, New Haven, CT) or IgG2a Isotype control (BioXCell; BP 00085) at 10 mg/kg i.p. at the dose schedule of Day -2, Day -1 prior to flank inoculation of cells, then again on Days 4, 8, and 11 post inoculation. .. Animals participating in the anti-TNF-α trial received injections of Abbvie synthesized anti-mu-TNF-α 8C11-1E10 (TNF-α mAb) or PBS at 15 mg/kg i.p. at the dose schedule of Day-2, Day -1 prior to flank inoculation of cells, then again on Days 4, 8, 11 and 15 post inoculation Complete response was predefined as three consecutive measurements of <25 mm 3 .

    Mouse Assay:

    Article Title: CD28-deficient mice are vulnerable to mouse papillomavirus MmuPV1 infection of the skin and mucosae
    Article Snippet: .. Mice were injected with either 300 μg of anti-mouse CD4 (clone GK1.5) or 300 μg of anti-mouse CD8 (clone 2.43 against CD8a) or both I.P. in 200 μl 1 × PBS at day 1, 2, 3 and CD80/CD86 (100 μg anti–B7-1 (16-10A1; BioXCell) and 100 μg anti–B7-1 (GL-1; BioXCell) every 2 days for up to 1 week before pre-scarification, and once weekly until sacrifice of the mice. .. Mice were injected with either 300 μg of anti-mouse CD4 (clone GK1.5) or 300 μg of anti-mouse CD8 (clone 2.43 against CD8a) or both I.P. in 200 μl 1 × PBS at day 1, 2, 3 and CD80/CD86 (100 μg anti–B7-1 (16-10A1; BioXCell) and 100 μg anti–B7-1 (GL-1; BioXCell) every 2 days for up to 1 week before pre-scarification, and once weekly until sacrifice of the mice.

    Injection:

    Article Title: CD28-deficient mice are vulnerable to mouse papillomavirus MmuPV1 infection of the skin and mucosae
    Article Snippet: .. Mice were injected with either 300 μg of anti-mouse CD4 (clone GK1.5) or 300 μg of anti-mouse CD8 (clone 2.43 against CD8a) or both I.P. in 200 μl 1 × PBS at day 1, 2, 3 and CD80/CD86 (100 μg anti–B7-1 (16-10A1; BioXCell) and 100 μg anti–B7-1 (GL-1; BioXCell) every 2 days for up to 1 week before pre-scarification, and once weekly until sacrifice of the mice. .. Mice were injected with either 300 μg of anti-mouse CD4 (clone GK1.5) or 300 μg of anti-mouse CD8 (clone 2.43 against CD8a) or both I.P. in 200 μl 1 × PBS at day 1, 2, 3 and CD80/CD86 (100 μg anti–B7-1 (16-10A1; BioXCell) and 100 μg anti–B7-1 (GL-1; BioXCell) every 2 days for up to 1 week before pre-scarification, and once weekly until sacrifice of the mice.

    Article Title: PARP inhibitors restore NK cell function via secretory crosstalk with tumor cells in prostate cancer
    Article Snippet: At 18 to 20 days after tumor injection, 150 mg/kg of d -luciferin, potassium salt (Yeasen) was injected into the abdominal cavity of the mice, and in vivo imaging analysis was performed 10 minutes after injection into the body. .. For in vivo depletion of immune cells, 200 μg per mouse of anti-mouse NK-1.1 (Bio X Cell) and anti-mouse CD8 (Bio X Cell) antibodies were administered intraperitoneally 2 days before tumor injection, followed by injections every 3 days. .. For in vivo CypA blockade, anti-CypA mAb (BGI Genomics) was intraperitoneally administered at a dose of 200 μg per mouse on day 1 after tumor inoculation, followed by weekly injections thereafter ( ).

    Article Title: Targeting tumor intrinsic TAK1 engages TNF-α-driven cell death through distinct mechanisms and enhances cancer immunotherapy
    Article Snippet: Where indicated, mice were treated with anti-mouse PD-1 antibody synthesized at AbbVie (17D2[mu IgG2a/k] DANA) 10 mg/kg, Q4Dx3, IP). .. For the CD8 depletion protocol, mice were injected with anti-mouse CD8 (BioXCell; BP0061, New Haven, CT) or IgG2a Isotype control (BioXCell; BP 00085) at 10 mg/kg i.p. at the dose schedule of Day -2, Day -1 prior to flank inoculation of cells, then again on Days 4, 8, and 11 post inoculation. .. Animals participating in the anti-TNF-α trial received injections of Abbvie synthesized anti-mu-TNF-α 8C11-1E10 (TNF-α mAb) or PBS at 15 mg/kg i.p. at the dose schedule of Day-2, Day -1 prior to flank inoculation of cells, then again on Days 4, 8, 11 and 15 post inoculation Complete response was predefined as three consecutive measurements of <25 mm 3 .

    other:

    Article Title: rWTC-MBTA Vaccine, Alone and Enhanced with Anti-PD1, Elicits Immune Responses against CNS and Peripheral B-Cell Lymphoma.
    Article Snippet: Antibodies, including anti-mouse CD40 (clone FGK4.5), anti-mouse CD4 (clone GK1.5), and anti-mouse CD8 (clone 53-6.7), were acquired from BioXCell (West Lebanon, NH).

    Bioprocessing:

    Article Title: Antibody-mediated conditioning with immunosuppression to enable allogeneic transplantation
    Article Snippet: .. Each recipient mouse received three consecutive 1 mg injections (days 0, 2 and 4 post-bone marrow infusion) of each of the following monoclonal antibodies: rat anti-mouse CD4 (CD4 cell non-depleting; YTS 177), anti-CD40 ligand (MR1) and anti-mouse CD8 (CD8 cell depleting; YTS 169), all from BioXcell (West Lebanon, NH). .. In addition, two doses of 12 mg/kg of rapamycin (LC Laboratories, Woburn, MA) were administered i.p. at days 6 and 30 post-bone marrow infusion.



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    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of <t>CD8</t> + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.
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    Image Search Results


    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

    Techniques: Over Expression, Cell Culture, Flow Cytometry, Co-culture Assay

    LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

    Techniques: In Vivo, Expressing, Quantitative RT-PCR, Analysis, Flow Cytometry, Immunofluorescence

    Combination drug treatment decreases tumor growth and increases plasma cytokine levels in humanized mice. A. Schematic representation of generation of humanized mice with a functioning immune system. B. Flow cytometry showing CD45, CD3, CD56, CD14, and CD16 expression in mice peripheral blood. C. Mice imaging showing tumor burden fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. D. Photon intensity on the whole mouse body fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. E. Mice body weight fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. F-H . Cytokine expression of F. TNFα, G. IFNγ, and H. CXCL10 respectively assessed by ELISA assay in plasma from retroorbital bleeds of euthanized mice 15 days after DAC (0.01 mg/kg), C92 (30 mg/kg), and combination treatment. I-J. Immunohistochemistry performed on spleen tissue showing I. CXCL10 cytokines and J. CD8 T cell infiltration respectively 15 days after DAC, C92, and combination treatment. K. Graphical model showing Necroptosis pathway activation by DAC and C92 STING agonist combination therapy in AML cells depleted of TP53. DAC and C92 combination therapy demonstrate increased TP53 independent STING pathway that leads to interferon transcription and triggers a ZNFX1-ZBP1 PANoptosis cell death pathway. Activated ZBP1 along with RIPK3 and MLKL phosphorylation are key features of necroptosis, with membrane rupture occurring as a part of this cell death pathway. In cell lines where ZNFX1 is depleted, ZBP1 is completely abrogated and necroptosis does not occur. All data are presented as mean +/- SEM with p-values derived from two-tailed unpaired Student’s t test or ANOVA as appropriate. * p<0.05, p<0.01, p<0.001, p<0.0001. All experiments were performed at least 3 times.

    Journal: bioRxiv

    Article Title: STING agonists in combination with epigenetic drugs potentiate ZNFX1-driven inflammatory necroptosis in TP53 -mutated AML

    doi: 10.64898/2026.07.27.741029

    Figure Lengend Snippet: Combination drug treatment decreases tumor growth and increases plasma cytokine levels in humanized mice. A. Schematic representation of generation of humanized mice with a functioning immune system. B. Flow cytometry showing CD45, CD3, CD56, CD14, and CD16 expression in mice peripheral blood. C. Mice imaging showing tumor burden fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. D. Photon intensity on the whole mouse body fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. E. Mice body weight fifteen days after post treatment with DAC (0.01 mg/kg), C92 (30 mg/kg) and combination. F-H . Cytokine expression of F. TNFα, G. IFNγ, and H. CXCL10 respectively assessed by ELISA assay in plasma from retroorbital bleeds of euthanized mice 15 days after DAC (0.01 mg/kg), C92 (30 mg/kg), and combination treatment. I-J. Immunohistochemistry performed on spleen tissue showing I. CXCL10 cytokines and J. CD8 T cell infiltration respectively 15 days after DAC, C92, and combination treatment. K. Graphical model showing Necroptosis pathway activation by DAC and C92 STING agonist combination therapy in AML cells depleted of TP53. DAC and C92 combination therapy demonstrate increased TP53 independent STING pathway that leads to interferon transcription and triggers a ZNFX1-ZBP1 PANoptosis cell death pathway. Activated ZBP1 along with RIPK3 and MLKL phosphorylation are key features of necroptosis, with membrane rupture occurring as a part of this cell death pathway. In cell lines where ZNFX1 is depleted, ZBP1 is completely abrogated and necroptosis does not occur. All data are presented as mean +/- SEM with p-values derived from two-tailed unpaired Student’s t test or ANOVA as appropriate. * p<0.05, p<0.01, p<0.001, p<0.0001. All experiments were performed at least 3 times.

    Article Snippet: Briefly, sections (5 μm) were deparaffinized, endogenous peroxidase was inactivated in 3% peroxide for 10 min, and antigen retrieval in 0.1 M sodium citrate was performed in a pressure cooker before the sections were blocked with 5% BSA and incubated overnight at 4 °C with polyclonal antibodies against CXCL10/IP-10 (Cat# 10937-1-AP, RRID:AB_2088002) and CD8 (Mouse anti-Human CD8 Antibody, Cat# 188-10258-IHC, RayBiotech, RRID:AB_2857849).

    Techniques: Clinical Proteomics, Flow Cytometry, Expressing, Imaging, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Activation Assay, Phospho-proteomics, Membrane, Derivative Assay, Two Tailed Test